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Figure 3.16 Typical commercial protein expression vectors. (a) pET-24a (+): the origin of replication (ori), kanamycin resistance (Kan; also known as KanR ), and the lactose repressor (lacI) are clearly shown. So too is the multiple cloning site (MCR) (illustration from www.merckbiosciences.com/docs/docs/PROT/TB070.pdf). (b) pET-11c: ori, lacI and MCR are clearly visible. So too is the ampicillin resistance (ApR ; also known as Amp, AmpR ) region. This protein expression vector also harbours a T7 promoter that acts as the promoter to drive the expression of heterologous DNA inserted at the MCR. Both maps have extensive restriction endonuclease mapping (illustration from www.merckbiosciences.com/docs/docs/PROT/TB042.pdf. 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